Tecan SNP Genotyping
Freedom EVO®|gDNA XLFreedom EVO® GenotypingLS Reloaded™ GenePaint™
Freedom EVO®|gDNA XL Automated solution for gDNA extraction from large volume samples based on Promega MagneSil® Genomic, Large Volume System The complete solution based on Freedom EVO|gDNA XL and Promega MagneSil® Genomic, Large Volume System is designed for scalable and automated genomic DNA isolation from large volume samples. The simple: direct lysis, bind, wash, and elute genomic DNA purification procedure allows purification of genomic DNA directly from a wide variety of sample types, including whole blood samples, regardless of their sample storage or shipping conditions. The purified genomic DNA is free from sample contaminates such as heme, protein and RNA, and is suitable for a variety of downstream applications such as: single and multiplex PCR, restriction digest, and real-time PCR. Highlights of Freedom EVO|gDNA XL are: Complete automation from whole blood samples to purified gDNA without user intervention On-the-fly scalability of chemistry Simultaneous processing of a wide range of sample volumes from 1 to 10ml Upgradeable. The Freedom EVO is reconfigurable and upgradeable for running additional experiment solutions. Centrifugation-less. Tecan and Promega developed the solution specifically to eliminate the need for centrifugation to increase reliability and decrease costs.
Freedom EVO® Genotyping - Scalable systems for medium to high-throughput SNP genotyping Tecan's Freedom EVO Genotyping solutions provide scalable, integrated systems for automated SNP genotyping. The ability to rapidly and efficiently screen genomic DNA samples for phenotypically-relevant SNPs (Single Nucleotide Polymorphisms) has become increasingly important to a wide range of disciplines including pharmacogenomics, clinical diagnostics, population genetics and forensic medicine. Freedom EVO Genotyping Workstation: Pre-optimized for all throughput demands for labs with higher throughput requirements, the Freedom EVO workstation has become the SNP screening platform of choice for many industry leaders. Freedom EVO can be configured with a wide range of modules and peripherals to allow for automation of almost any procedure, efficiently performing steps such as: DNA isolation and quantitation PCR/sequencing reaction set-up microplate sealing amplification/sequencing (by integration of third party thermocyclers) PCR/sequencing clean-up assay detection data analysis
LS Reloaded™ Use any microarray substrate...but select the right scanner Automation-friendly high sensitivity scanning platform for your microarray formats Accelerated and reliable microarray analysis: With its automation-ready design, parallel dual channel detection capability, and ability to operate up to 4 excitation lasers and 28 emission filters, Tecan's LS Reloaded Scanner greatly accelerates fluorescence laser scanning. Increased throughput and multiplexing capabilities for array and other micro feature scanning and analysis mean savings in both time and resources. Compatible with standard 3x1 inch slides for customized chips and microplates Three different grades of confocal measurements Autofocus system for planar adjustment with capability to scan through glass Spatial scanning resolution from 4 to 40 µm 4-color scanning to increase multiplexing capability Scattered light scanning capability to detect insufficient spot quality prior to hybridization without dyes Adjustable angle of laser beam for dual colour scanning of Evanescence Resonance slides increases Cy3/Cy5 sensitivity 10-fold LaserCheck™ assists in verifying and documenting the consistent performance of LS 4-slide adapter provides seamless integration of HS 400/4800™ hybridization station and Connect™ autoloader with LS Reloaded. Connect autoloader provides scalable throughput for batch processing of up to 200 slides or 50 microplates Array-Pro® Analyzer provides every conceivable image analysis tool to characterize the measurement system and achieve maximum signal/noise ratios, repeatability of results and quantitative accuracy
GenePaint™ Innovative solution for automated in situ hybridization (ISH) flourescence in situ hybridization (FISH) and immunohistochemistry (IHC) Tecan's GenePaint system, which is based on a new flow-through technology, automates all of the steps required for performing in situ hybridization of tissues on microscope slide formats. One of the key steps in the study of gene expression is the validation of results obtained by in vitro gene expression methods (microarrays, blots, etc.). This validation typically involves the use of appropriate cell or tissue samples and the performance of in situ hybridization (ISH) to confirm the in vitro results. The unique advantage of in situ hybridization over destructive expression techniques such as microarrays is the localization of transcripts in intact cells and tissues, giving a much higher level of information. However, processing of large numbers of cell or tissue samples in these type of procedures is an extremely time consuming and laborious process. GenePaint alleviates this throughput bottleneck. Advanced features for functional genomic studies eliminates 80% of the manual steps of conventional ISH, FISH or IHC analysis extends walk-away time through unattended hybridization protocols allows the use of any commercial set of reagents for hybridization studies assures consistent and reproducible results of excellent quality saves on the amounts of reagents used
Live Presentation 12 December 2005 (archive available) Marc Feiglin:SNP Genotyping
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